GHK-Cu Reconstitution & Dosing

Also known as: Copper peptide

Quick answer: mix a 50 mg vial of GHK-Cu with 5 ml of bacteriostatic water. That gives 10.00 mg/ml, so a typical 1 mg dose is 10 units on a U-100 insulin syringe — about 50 doses per vial. Reported protocols range 1 mg2 mg, daily.

GHK-Cu is a naturally occurring copper-binding tripeptide studied for skin remodeling, collagen synthesis and wound healing. It is used both as a subcutaneous injection and in topical cosmetic formulations. Full overview: what is GHK-Cu? →

Calculate your exact dose

Typical range: 1 mg2 mg, daily

10 units
to draw on a U-100 insulin syringe (0.10 ml)
Concentration: 10.00 mg/ml (100.0 mcg per unit)
Doses per vial: 50
020406080100
Syringe fill (100-unit / 1 ml insulin syringe)

GHK-Cu mixing chart — 50 mg vial

Units to draw for a 1 mg dose at different water amounts. More water never changes the dose — only the volume you measure.

Water addedConcentration1 mg dose =Per unit
1 ml 50.00 mg/ml2.0 units500.0 mcg
2 ml 25.00 mg/ml4.0 units250.0 mcg
2.5 ml 20.00 mg/ml5.0 units200.0 mcg
3 ml 16.67 mg/ml6.0 units166.7 mcg
5 ml ← typical10.00 mg/ml10 units100.0 mcg

Protocol reference

Common vial sizes
50 mg, 100 mg
Reported dose range
1 mg – 2 mg
Frequency
daily
Administration
subcutaneous or topical
Storage
Reconstituted: refrigerate. Solution is naturally blue from the copper complex.

FAQ

How much bacteriostatic water do you add to GHK-Cu?+

Most protocols mix a 50 mg vial of GHK-Cu with 5 ml of bacteriostatic water. This gives 10.00 mg/ml, so a typical 1 mg dose is 10 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.

What is a typical GHK-Cu dose?+

Commonly reported GHK-Cu protocols use 1 mg to 2 mg, daily, administered via subcutaneous or topical. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.

How many doses are in a 50 mg vial of GHK-Cu?+

At 1 mg per dose, a 50 mg vial contains about 50 doses, regardless of how much water you add.

How should GHK-Cu be stored?+

Reconstituted: refrigerate. Solution is naturally blue from the copper complex.

Related peptides

Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. GHK-Cu may not be approved for human use in your jurisdiction.