TB-500 Reconstitution & Dosing
Also known as: Thymosin Beta-4, TB4
Quick answer: mix a 5 mg vial of TB-500 with 2.5 ml of bacteriostatic water. That gives 2.00 mg/ml, so a typical 2.5 mg dose is 125 units on a U-100 insulin syringe — about 2 doses per vial. Reported protocols range 2 mg–5 mg, 2x weekly (loading), then 1x weekly.
TB-500 is the synthetic version of Thymosin Beta-4, a naturally occurring repair peptide, studied for soft-tissue recovery, flexibility and reduced inflammation. Protocols typically front-load twice weekly for 4–6 weeks before dropping to maintenance. Full overview: what is TB-500? →
Calculate your exact dose
Typical range: 2 mg–5 mg, 2x weekly (loading), then 1x weekly
⚠ This dose needs more than one full 1 ml syringe (1.25 ml). Use less water when reconstituting, or split into multiple injections.
TB-500 mixing chart — 5 mg vial
Units to draw for a 2.5 mg dose at different water amounts. More water never changes the dose — only the volume you measure.
| Water added | Concentration | 2.5 mg dose = | Per unit |
|---|---|---|---|
| 1 ml | 5.00 mg/ml | 50 units | 50.0 mcg |
| 2 ml | 2.50 mg/ml | 100 units | 25.0 mcg |
| 2.5 ml ← typical | 2.00 mg/ml | 125 units (>1 syringe) | 20.0 mcg |
| 3 ml | 1.67 mg/ml | 150 units (>1 syringe) | 16.7 mcg |
| 5 ml | 1.00 mg/ml | 250 units (>1 syringe) | 10.0 mcg |
Protocol reference
FAQ
How much bacteriostatic water do you add to TB-500?+
Most protocols mix a 5 mg vial of TB-500 with 2.5 ml of bacteriostatic water. This gives 2.00 mg/ml, so a typical 2.5 mg dose is 125 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.
What is a typical TB-500 dose?+
Commonly reported TB-500 protocols use 2 mg to 5 mg, 2x weekly (loading), then 1x weekly, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.
How many doses are in a 5 mg vial of TB-500?+
At 2.5 mg per dose, a 5 mg vial contains about 2 doses, regardless of how much water you add.
How should TB-500 be stored?+
Reconstituted: refrigerate, typically used within 4–6 weeks.
Related peptides
Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. TB-500 may not be approved for human use in your jurisdiction.