LL-37 Reconstitution & Dosing

Also known as: Cathelicidin

Quick answer: mix a 5 mg vial of LL-37 with 2.5 ml of bacteriostatic water. That gives 2.00 mg/ml, so a typical 100 mcg dose is 5.0 units on a U-100 insulin syringe — about 50 doses per vial. Reported protocols range 50 mcg100 mcg, daily.

LL-37 is the only human cathelicidin antimicrobial peptide, studied for biofilm disruption and immune modulation. Reported research doses are small — most protocols stay at or under 100 mcg daily. Full overview: what is LL-37? →

Calculate your exact dose

Typical range: 50 mcg100 mcg, daily

5.0 units
to draw on a U-100 insulin syringe (0.05 ml)
Concentration: 2.00 mg/ml (20.0 mcg per unit)
Doses per vial: 50
020406080100
Syringe fill (100-unit / 1 ml insulin syringe)

LL-37 mixing chart — 5 mg vial

Units to draw for a 100 mcg dose at different water amounts. More water never changes the dose — only the volume you measure.

Water addedConcentration100 mcg dose =Per unit
1 ml 5.00 mg/ml2.0 units50.0 mcg
2 ml 2.50 mg/ml4.0 units25.0 mcg
2.5 ml ← typical2.00 mg/ml5.0 units20.0 mcg
3 ml 1.67 mg/ml6.0 units16.7 mcg
5 ml 1.00 mg/ml10 units10.0 mcg

Protocol reference

Common vial sizes
5 mg
Reported dose range
50 mcg – 100 mcg
Frequency
daily
Administration
subcutaneous injection
Storage
Reconstituted: refrigerate, typically used within 4 weeks.

FAQ

How much bacteriostatic water do you add to LL-37?+

Most protocols mix a 5 mg vial of LL-37 with 2.5 ml of bacteriostatic water. This gives 2.00 mg/ml, so a typical 100 mcg dose is 5.0 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.

What is a typical LL-37 dose?+

Commonly reported LL-37 protocols use 50 mcg to 100 mcg, daily, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.

How many doses are in a 5 mg vial of LL-37?+

At 100 mcg per dose, a 5 mg vial contains about 50 doses, regardless of how much water you add.

How should LL-37 be stored?+

Reconstituted: refrigerate, typically used within 4 weeks.

Related peptides

Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. LL-37 may not be approved for human use in your jurisdiction.