Thymosin Alpha-1 Reconstitution & Dosing

Also known as: Tα1, Zadaxin

Quick answer: mix a 5 mg vial of Thymosin Alpha-1 with 2 ml of bacteriostatic water. That gives 2.50 mg/ml, so a typical 1.6 mg dose is 64 units on a U-100 insulin syringe — about 3 doses per vial. Reported protocols range 450 mcg1.6 mg, 2x weekly.

Thymosin Alpha-1 is a thymic peptide approved in several countries as an immune stimulant (Zadaxin) for hepatitis and as a vaccine adjuvant. The clinically studied dose is 1.6 mg twice weekly. Full overview: what is Thymosin Alpha-1? →

Calculate your exact dose

Typical range: 450 mcg1.6 mg, 2x weekly

64 units
to draw on a U-100 insulin syringe (0.64 ml)
Concentration: 2.50 mg/ml (25.0 mcg per unit)
Doses per vial: 3
020406080100
Syringe fill (100-unit / 1 ml insulin syringe)

Thymosin Alpha-1 mixing chart — 5 mg vial

Units to draw for a 1.6 mg dose at different water amounts. More water never changes the dose — only the volume you measure.

Water addedConcentration1.6 mg dose =Per unit
1 ml 5.00 mg/ml32 units50.0 mcg
2 ml ← typical2.50 mg/ml64 units25.0 mcg
2.5 ml 2.00 mg/ml80 units20.0 mcg
3 ml 1.67 mg/ml96 units16.7 mcg
5 ml 1.00 mg/ml160 units (>1 syringe)10.0 mcg

Protocol reference

Common vial sizes
5 mg, 10 mg
Reported dose range
450 mcg – 1.6 mg
Frequency
2x weekly
Administration
subcutaneous injection
Storage
Reconstituted: refrigerate, typically used within 4 weeks.

FAQ

How much bacteriostatic water do you add to Thymosin Alpha-1?+

Most protocols mix a 5 mg vial of Thymosin Alpha-1 with 2 ml of bacteriostatic water. This gives 2.50 mg/ml, so a typical 1.6 mg dose is 64 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.

What is a typical Thymosin Alpha-1 dose?+

Commonly reported Thymosin Alpha-1 protocols use 450 mcg to 1.6 mg, 2x weekly, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.

How many doses are in a 5 mg vial of Thymosin Alpha-1?+

At 1.6 mg per dose, a 5 mg vial contains about 3 doses, regardless of how much water you add.

How should Thymosin Alpha-1 be stored?+

Reconstituted: refrigerate, typically used within 4 weeks.

Related peptides

Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. Thymosin Alpha-1 may not be approved for human use in your jurisdiction.