NAD+ Reconstitution Calculator & Dosage Chart
Also known as: Nicotinamide adenine dinucleotide
In short: mix a 500 mg vial of NAD+ with 5 ml of bacteriostatic water. That gives 100.00 mg/ml, so a typical 50 mg dose is 50 units on a U-100 insulin syringe — about 10 doses per vial. Reported protocols range 50 mg–100 mg, 1–3x weekly.
NAD+ is the central coenzyme of cellular energy metabolism, injected subcutaneously in longevity protocols as levels decline with age. Doses are in milligrams (50–100 mg), far larger than typical peptides — get the math right. Full overview: what is NAD+? →
Calculate your exact dose
Typical range: 50 mg–100 mg, 1–3x weekly
NAD+ mixing chart — 500 mg vial
Units to draw for a 50 mg dose at different water amounts. More water never changes the dose — only the volume you measure.
| Water added | Concentration | 50 mg dose = | Per unit |
|---|---|---|---|
| 1 ml | 500.00 mg/ml | 10 units | 5000.0 mcg |
| 2 ml | 250.00 mg/ml | 20 units | 2500.0 mcg |
| 2.5 ml | 200.00 mg/ml | 25 units | 2000.0 mcg |
| 3 ml | 166.67 mg/ml | 30 units | 1666.7 mcg |
| 5 ml ← typical | 100.00 mg/ml | 50 units | 1000.0 mcg |
NAD+ reconstitution by vial size — 100 mg / 500 mg / 1000 mg
How much water for each vial size, and what a 50 mg dose becomes in syringe units. Bigger vials hold more doses; they do not change the dose.
| Vial | Water | Concentration | 50 mg dose = | Doses per vial |
|---|---|---|---|---|
| 100 mg | 2 ml | 50.00 mg/ml | 100 units | 2 |
| 100 mg | 3 ml | 33.33 mg/ml | 150 units (>1 syringe) | 2 |
| 100 mg | 5 ml | 20.00 mg/ml | 250 units (>1 syringe) | 2 |
| 500 mg | 2 ml | 250.00 mg/ml | 20 units | 10 |
| 500 mg | 3 ml | 166.67 mg/ml | 30 units | 10 |
| 500 mg | 5 ml | 100.00 mg/ml | 50 units | 10 |
| 1000 mg | 2 ml | 500.00 mg/ml | 10 units | 20 |
| 1000 mg | 3 ml | 333.33 mg/ml | 15 units | 20 |
| 1000 mg | 5 ml | 200.00 mg/ml | 25 units | 20 |
Protocol reference
FAQ
How much bacteriostatic water do you add to NAD+?+
Most protocols mix a 500 mg vial of NAD+ with 5 ml of bacteriostatic water. This gives 100.00 mg/ml, so a typical 50 mg dose is 50 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.
What is a typical NAD+ dose?+
Commonly reported NAD+ protocols use 50 mg to 100 mg, 1–3x weekly, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.
How many doses are in a 500 mg vial of NAD+?+
At 50 mg per dose, a 500 mg vial contains about 10 doses, regardless of how much water you add.
How should NAD+ be stored?+
Reconstituted: refrigerate, protect from light, use within 2–4 weeks.
Related peptides
Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. NAD+ may not be approved for human use in your jurisdiction.