SS-31 Reconstitution & Dosing
Also known as: Elamipretide
Quick answer: mix a 10 mg vial of SS-31 with 2 ml of bacteriostatic water. That gives 5.00 mg/ml, so a typical 10 mg dose is 200 units on a U-100 insulin syringe — about 1 doses per vial. Reported protocols range 5 mg–20 mg, daily or every other day.
SS-31 (elamipretide) is a mitochondria-targeted peptide that binds cardiolipin in the inner mitochondrial membrane, studied in clinical trials for mitochondrial myopathy and heart failure. Full overview: what is SS-31? →
Calculate your exact dose
Typical range: 5 mg–20 mg, daily or every other day
⚠ This dose needs more than one full 1 ml syringe (2.00 ml). Use less water when reconstituting, or split into multiple injections.
SS-31 mixing chart — 10 mg vial
Units to draw for a 10 mg dose at different water amounts. More water never changes the dose — only the volume you measure.
| Water added | Concentration | 10 mg dose = | Per unit |
|---|---|---|---|
| 1 ml | 10.00 mg/ml | 100 units | 100.0 mcg |
| 2 ml ← typical | 5.00 mg/ml | 200 units (>1 syringe) | 50.0 mcg |
| 2.5 ml | 4.00 mg/ml | 250 units (>1 syringe) | 40.0 mcg |
| 3 ml | 3.33 mg/ml | 300 units (>1 syringe) | 33.3 mcg |
| 5 ml | 2.00 mg/ml | 500 units (>1 syringe) | 20.0 mcg |
Protocol reference
FAQ
How much bacteriostatic water do you add to SS-31?+
Most protocols mix a 10 mg vial of SS-31 with 2 ml of bacteriostatic water. This gives 5.00 mg/ml, so a typical 10 mg dose is 200 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.
What is a typical SS-31 dose?+
Commonly reported SS-31 protocols use 5 mg to 20 mg, daily or every other day, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.
How many doses are in a 10 mg vial of SS-31?+
At 10 mg per dose, a 10 mg vial contains about 1 doses, regardless of how much water you add.
How should SS-31 be stored?+
Reconstituted: refrigerate, typically used within 4 weeks.
Related peptides
Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. SS-31 may not be approved for human use in your jurisdiction.