MOTS-c Reconstitution & Dosing

Quick answer: mix a 10 mg vial of MOTS-c with 2 ml of bacteriostatic water. That gives 5.00 mg/ml, so a typical 5 mg dose is 100 units on a U-100 insulin syringe — about 2 doses per vial. Reported protocols range 5 mg10 mg, 1–3x weekly.

MOTS-c is a mitochondrial-derived peptide studied for exercise capacity, insulin sensitivity and metabolic regulation — often described as an 'exercise mimetic' in the research literature. Full overview: what is MOTS-c? →

Calculate your exact dose

Typical range: 5 mg10 mg, 1–3x weekly

100 units
to draw on a U-100 insulin syringe (1.00 ml)
Concentration: 5.00 mg/ml (50.0 mcg per unit)
Doses per vial: 2
020406080100
Syringe fill (100-unit / 1 ml insulin syringe)

MOTS-c mixing chart — 10 mg vial

Units to draw for a 5 mg dose at different water amounts. More water never changes the dose — only the volume you measure.

Water addedConcentration5 mg dose =Per unit
1 ml 10.00 mg/ml50 units100.0 mcg
2 ml ← typical5.00 mg/ml100 units50.0 mcg
2.5 ml 4.00 mg/ml125 units (>1 syringe)40.0 mcg
3 ml 3.33 mg/ml150 units (>1 syringe)33.3 mcg
5 ml 2.00 mg/ml250 units (>1 syringe)20.0 mcg

Protocol reference

Common vial sizes
5 mg, 10 mg
Reported dose range
5 mg – 10 mg
Frequency
1–3x weekly
Administration
subcutaneous injection
Storage
Reconstituted: refrigerate, typically used within 4 weeks.

FAQ

How much bacteriostatic water do you add to MOTS-c?+

Most protocols mix a 10 mg vial of MOTS-c with 2 ml of bacteriostatic water. This gives 5.00 mg/ml, so a typical 5 mg dose is 100 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.

What is a typical MOTS-c dose?+

Commonly reported MOTS-c protocols use 5 mg to 10 mg, 1–3x weekly, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.

How many doses are in a 10 mg vial of MOTS-c?+

At 5 mg per dose, a 10 mg vial contains about 2 doses, regardless of how much water you add.

How should MOTS-c be stored?+

Reconstituted: refrigerate, typically used within 4 weeks.

Related peptides

Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. MOTS-c may not be approved for human use in your jurisdiction.