VIP (Vasoactive Intestinal Peptide) Reconstitution Calculator & Dosage Chart

Also known as: Aviptadil, VIP nasal spray

Quick answer: mix a 5 mg vial of VIP (Vasoactive Intestinal Peptide) with 2 ml of bacteriostatic water. That gives 2.50 mg/ml, so a typical 50 mcg dose is 2.0 units on a U-100 insulin syringe — about 100 doses per vial. Reported protocols range 50 mcg200 mcg, 1–4x daily (nasal protocols use 50 mcg per spray).

VIP is a 28-amino-acid neuropeptide with broad anti-inflammatory, vasodilatory and immune-regulating effects. It is best known as the final step of the Shoemaker CIRS (mold illness) protocol as a compounded nasal spray, and as aviptadil in respiratory research. Full overview: what is VIP (Vasoactive Intestinal Peptide)? →

Calculate your exact dose

Typical range: 50 mcg200 mcg, 1–4x daily (nasal protocols use 50 mcg per spray)

2.0 units
to draw on a U-100 insulin syringe (0.02 ml)
Concentration: 2.50 mg/ml (25.0 mcg per unit)
Doses per vial: 100
020406080100
Syringe fill (100-unit / 1 ml insulin syringe)

VIP (Vasoactive Intestinal Peptide) mixing chart — 5 mg vial

Units to draw for a 50 mcg dose at different water amounts. More water never changes the dose — only the volume you measure.

Water addedConcentration50 mcg dose =Per unit
1 ml 5.00 mg/ml1.0 units50.0 mcg
2 ml ← typical2.50 mg/ml2.0 units25.0 mcg
2.5 ml 2.00 mg/ml2.5 units20.0 mcg
3 ml 1.67 mg/ml3.0 units16.7 mcg
5 ml 1.00 mg/ml5.0 units10.0 mcg

VIP (Vasoactive Intestinal Peptide) reconstitution by vial size — 2 mg / 5 mg / 6 mg

How much water for each vial size, and what a 50 mcg dose becomes in syringe units. Bigger vials hold more doses; they do not change the dose.

VialWaterConcentration50 mcg dose =Doses per vial
2 mg1 ml2.00 mg/ml2.5 units40
2 mg2 ml1.00 mg/ml5.0 units40
2 mg3 ml0.67 mg/ml7.5 units40
5 mg1 ml5.00 mg/ml1.0 units100
5 mg2 ml2.50 mg/ml2.0 units100
5 mg3 ml1.67 mg/ml3.0 units100
6 mg1 ml6.00 mg/ml0.8 units120
6 mg2 ml3.00 mg/ml1.7 units120
6 mg3 ml2.00 mg/ml2.5 units120

Protocol reference

Common vial sizes
2 mg, 5 mg, 6 mg
Reported dose range
50 mcg – 200 mcg
Frequency
1–4x daily (nasal protocols use 50 mcg per spray)
Administration
intranasal or subcutaneous
Storage
Reconstituted: refrigerate; VIP is fragile — protect from heat and use within 2–3 weeks.

FAQ

How much bacteriostatic water do you add to VIP (Vasoactive Intestinal Peptide)?+

Most protocols mix a 5 mg vial of VIP (Vasoactive Intestinal Peptide) with 2 ml of bacteriostatic water. This gives 2.50 mg/ml, so a typical 50 mcg dose is 2.0 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.

What is a typical VIP (Vasoactive Intestinal Peptide) dose?+

Commonly reported VIP (Vasoactive Intestinal Peptide) protocols use 50 mcg to 200 mcg, 1–4x daily (nasal protocols use 50 mcg per spray), administered via intranasal or subcutaneous. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.

How many doses are in a 5 mg vial of VIP (Vasoactive Intestinal Peptide)?+

At 50 mcg per dose, a 5 mg vial contains about 100 doses, regardless of how much water you add.

How should VIP (Vasoactive Intestinal Peptide) be stored?+

Reconstituted: refrigerate; VIP is fragile — protect from heat and use within 2–3 weeks.

Related peptides

Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. VIP (Vasoactive Intestinal Peptide) may not be approved for human use in your jurisdiction.