Humanin Reconstitution Calculator & Dosage Chart
Also known as: HN, Mitochondrial-derived peptide
Quick answer: mix a 10 mg vial of Humanin with 2 ml of bacteriostatic water. That gives 5.00 mg/ml, so a typical 2 mg dose is 40 units on a U-100 insulin syringe — about 5 doses per vial. Reported protocols range 1 mg–4 mg, 2–3x weekly.
Humanin is a 24-amino-acid peptide encoded inside mitochondrial DNA — the first of the mitochondrial-derived peptides, alongside MOTS-c. It is studied for cytoprotection, insulin sensitivity and neuroprotection; circulating levels fall with age and are elevated in centenarians' offspring. Full overview: what is Humanin? →
Calculate your exact dose
Typical range: 1 mg–4 mg, 2–3x weekly
Humanin mixing chart — 10 mg vial
Units to draw for a 2 mg dose at different water amounts. More water never changes the dose — only the volume you measure.
| Water added | Concentration | 2 mg dose = | Per unit |
|---|---|---|---|
| 1 ml | 10.00 mg/ml | 20 units | 100.0 mcg |
| 2 ml ← typical | 5.00 mg/ml | 40 units | 50.0 mcg |
| 2.5 ml | 4.00 mg/ml | 50 units | 40.0 mcg |
| 3 ml | 3.33 mg/ml | 60 units | 33.3 mcg |
| 5 ml | 2.00 mg/ml | 100 units | 20.0 mcg |
Humanin reconstitution by vial size — 5 mg / 10 mg
How much water for each vial size, and what a 2 mg dose becomes in syringe units. Bigger vials hold more doses; they do not change the dose.
| Vial | Water | Concentration | 2 mg dose = | Doses per vial |
|---|---|---|---|---|
| 5 mg | 1 ml | 5.00 mg/ml | 40 units | 2 |
| 5 mg | 2 ml | 2.50 mg/ml | 80 units | 2 |
| 5 mg | 3 ml | 1.67 mg/ml | 120 units (>1 syringe) | 2 |
| 10 mg | 1 ml | 10.00 mg/ml | 20 units | 5 |
| 10 mg | 2 ml | 5.00 mg/ml | 40 units | 5 |
| 10 mg | 3 ml | 3.33 mg/ml | 60 units | 5 |
Protocol reference
FAQ
How much bacteriostatic water do you add to Humanin?+
Most protocols mix a 10 mg vial of Humanin with 2 ml of bacteriostatic water. This gives 5.00 mg/ml, so a typical 2 mg dose is 40 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.
What is a typical Humanin dose?+
Commonly reported Humanin protocols use 1 mg to 4 mg, 2–3x weekly, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.
How many doses are in a 10 mg vial of Humanin?+
At 2 mg per dose, a 10 mg vial contains about 5 doses, regardless of how much water you add.
How should Humanin be stored?+
Reconstituted: refrigerate, use within 4 weeks.
Related peptides
Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. Humanin may not be approved for human use in your jurisdiction.